Not known Details About attune flow cytometer
Not known Details About attune flow cytometer
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Details-enriched characterization of cell populations. Morphological facts from visuals can add for the richness of flow cytometry data. For example, the figure exhibits an if not regular apoptosis assay making use of Annexin V and PI, including cell imaging to characterize cells in Each and every populace to reveal morphologically unique options. These insights could not have already been gained from multiplex staining by itself.
As samples are obtained around the Attune CytPix Flow Cytometer, the high-velocity brightfield digicam captures and suppliers illustrations or photos of detected occasions at a fee approximately six,000 images for every second, with regards to the flow amount and picture dimension. For larger versatility, Attune Cytometric Software program enables you to change the picture capture frequency as required and choose a specific gate for image capture, if expected.
(B) Uncooked 264.7 macrophages will often be annotated inaccurately with respect to particle rely because of their complexity and noncircular borders. The amount of singlets obtainable for Investigation improves as soon as the consumer trains and employs a far more accurate design.
The award-winning Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing having a substantial-pace brightfield camera for simultaneous large throughput flow cytometry and substantial resolution brightfield imaging.
"A picture isn't fairly really worth 1000 dots, but it surely genuinely helps you to see photos. It is excellent to discover images that correlate with staining. As an example, AnnexinV-constructive cells which can be morphologically distinct than wholesome cells, or CD14+ monocytes which can be bigger and even more textured than CD3+/CD4+/CD14- lymphocytes.
Right here, a highly skilled consumer has gated singlets confidently. Following analyzing the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate contains much more than four% aggregates.
Optimizing gating technique. Even strong guide singlet gating is error-inclined and stays a subjective decision position in almost all flow cytometry assays. Imaging can be utilized to verify and alter gates to include only solitary cells of fascination.
Consistent picture excellent even at significant throughput Acoustic focusing and a large-speed digital camera Incorporate to picture these T cells consistently at small or large flow costs. Easily change concentrate and camera options to fulfill experimental necessities.
The Attune NxT cytometer may be the reputable workhorse instrument your lab can trust in for consistent and reliable performance. The Attune CytPix design incorporates a high-pace brightfield digicam and AI-driven image Assessment software program, with current updates together with equipment Finding out resources and an intuitive consumer interface, enabling enhanced data precision, accuracy, and speedier insights into sample biology.
Users can watch function illustrations or photos in serious-time to confirm sample quality, enhance protocols, and record the morphology of each and every mobile population determined for Assessment. Imaging capture also Advantages from acoustic focusing, which really helps to situation the cells making sure that a pointy, centered picture is obtained.
Sound waves empower specific mobile here alignment, resulting in Outstanding information top quality and ~10X faster sample flow charges.
Application note: Automated image Investigation minimizes person-to-person variability in flow cytometry gating techniques
Leverage the power of Sophisticated technological innovation and innovation to speed up your discoveries. Trust within the Attune Flow Cytometer household to deliver the dependability, speed, and accuracy that established your perform apart.
Versatile payment approach from Agilent Financial Solutions assist you to obtain the essential instrumentation you require for speedy challenge, or to grow your lab.
Gating on these extended parameters means that you can promptly and correctly establish populations of interest and ensure gating system with little if any handbook assessment. The Attune software package presents access to state-of-the-artwork device Mastering Assessment resources inside an expertly created consumer interface, rendering it exceptionally user friendly.
Since the mobile line experienced Beforehand been analyzed within the Attune CytPix Flow Cytometer, the researchers went back again to the pictures and were being capable to document the microbial an infection a minimum of 5 times earlier.
The Attune CytPix Flow Cytometer makes it possible for users to visualise samples through acquisition, confirm the precision of gating techniques, and help outcomes with dependable documentation for publication purposes.
In this article, we display that by gating tactics utilizing graphic-dependent quantitative parameters can distinguish interacting cells from coincident events to much more accurately assess interacting cells.